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出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5 测序引物:5 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET32c, pet 32c
大肠杆菌蛋白表达
高
多克隆位点，限制性内切酶
5901bp
T7
5-TAATACGACTCACTATAGGG-3
thioredoxin (N端); His (中间和C端)
Ampicillin
Production of soluble, active target proteins; N-term thrombin cleavage site;
Nterm enterokinase cleavage site; a,b,c vary by MCS
69017-3
瞬时表达 Transient
组成型 Constitutive
非病毒
pET32c载体质粒图谱和多克隆位点信息

pET32c载体简介
The pET-32 series is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pET32c载体序列
LOCUS       pET-32c(+)              5901 bp    DNA     circular     11-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     promoter        complement(765..781)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_signal     complement(367..693)
                     /vntifkey="87"
                     /label=trx\tag
     protein_bind    complement(328..345)
                     /vntifkey="31"
                     /label=His\tag
     misc_feature    complement(250..294)
                     /vntifkey="21"
                     /label=S\tag
     protein_bind    complement(140..157)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             1172..2251
                     /vntifkey="4"
                     /label=lac\I
     rep_origin      complement(3685..3685)
                     /vntifkey="33"
                     /label=ColE1\pBR322
     CDS             complement(4446..5303)
                     /vntifkey="4"
                     /label=bla\(amp)\sequence
     rep_origin      complement(5435..5890)
                     /vntifkey="33"
                     /label=f1\origin
     CDS             complement(738..762)
                     /vntifkey="4"
                     /label=lac\operator
     protein_bind    complement(301..318)
                     /vntifkey="31"
                     /label=thrombin
     protein_bind    complement(220..234)
                     /vntifkey="31"
                     /label=enterokinase
BASE COUNT     1392 a      1565 c      1559 g      1385 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc
      241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc
      301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc
      361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac
      421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc
      481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc
      541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca
      601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact
      661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac
      721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt
      781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
     1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
     1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
   &