预染蛋白marker 10-170KDa(26616)
产品名称: 预染蛋白marker 10-170KDa(26616)
英文名称: PageRuler Prest Protein Ladder
产品编号: SM0671/26616
产品价格: 0
产品产地: fermentas
品牌商标: fermentas
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 湖北省武汉市洪山区珞狮南路517号明泽大厦1006室
- 邮编 : 430070
- 所在区域 : 湖北
- 电话 : 131****6941
- 传真 : 027-88185565
- 邮箱 : 2248475041@qq.com
Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 170kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.
Highlights:
- Size range – 10 proteins spanning 10 to 170kDa
- Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
- Sharp bands – color-coded bands of similar intensity for easy visualization
- Quality tested – each lot evaluated by SDS-PAGE and Western blotting
- Two reference bands – orange at 70kDa and green at 10kDa
- Membrane-compatible – colored bands transfer to membranes for Western blotting
Includes:
- Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Applications:
- Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring protein transfer onto membranes after Western blotting
- Sizing of proteins on SDS-polyacrylamide gels and Western blots
Product Details:
SDS-PAGE band profile of the Thermo Scientific PageRuler Prestained Protein Ladder. Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane. |