多功能干细胞神经诱导培养基上海中乔新舟生物科技有限公司

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产品名称: 多功能干细胞神经诱导培养基
英文名称: HPSC Neural Induction Medium
产品货号: 5931
产品规格: 500ml
产品产地: 美国
产品商标: sciencell
价    格: 询价元
保存与运输: 询价
产品资料: 实验方法技术资料
更新时间: 2020/3/16 10:52:00
浏览人数:421
诚信指数:1262点
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使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

上海中乔新舟生物科技有限公司
地址:
上海市宝山区长江南路180号A区401-406室
邮编:
200433
电话:
021-56145703/18117540148
传真:
021-56760351
联系人:
胡小姐
所在区域:
上海·上海
邮件:
公司展台:
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产品详细描述

Description
Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) possess the capability of differentiating into all derivatives of the three germ layers including neural cells. Human pluripotent stem cell (hPSC) derived neural cells have been used for studying nervous system development, modeling neurologic disorders, neurotoxicology and drug screening. SMAD signaling plays a crucial role in neural induction. Dual inhibition of SMAD signaling by Noggin and SB431542 is sufficient to induce rapid and complete neural conversion of hPSCs [1]. In 2010, Wenlin Li and colleagues found that synergistic inhibition of glycogen synthase kinase 3 (GSK3) and transforming growth factor β (TGF-β) could efficiently differentiate hPSCs to homogenous neural epithelium in 7 days [2]. The derived neural stem cells (NSCs) can self-renew in the presence of human leukemia inhibitory factor (LIF) and inhibitors of GSK3 and TGF-β. NSCs possess the multipotential of differentiating into various neuronal and glial subtypes upon induction with specific patterning cues [2]. 

 
Based on the advances in neural differentiation methodologies, we developed hPSC Neural Induction Medium (PSCNIM, Cat. #5931), a serum-free medium, to efficiently convert hPSCs to NSCs under adherent culture conditions. The hPSCs are converted to homogenous neural epithelium using neural induction medium (Figure 1). After a 7 – 10 day treatment with PSCNIM, more than 90% of cells expressed the neural stem cell markers: Nestin and Sox2; while the pluripotency marker Nanog expression was lost (Figure 2). NSCs derived from hPSC can be maintained in the induction medium for at least 5 passages (~24 days), can efficiently form rosettes (Figure 3) and be expanded as neural progenitor cells in EGF and FGF containing medium, or differentiated into neural lineages such as neurons and astrocytes (Figure 3).

 
Components
Neural induction basal medium (Cat. #5931-b) - 100 mL x 5
Neural induction medium supplement 50x (Cat. #5982) - 2 mL x 5

 
Storage
Store the neural induction basal medium at 4°C and the neural induction medium supplement (50x) at -20°C. Protect from light.

 
Shipping
Neural induction medium supplement (50x) is shipped on dry ice. Neural induction basal medium is shipped at 4°C.

 
Reference
[1] Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. (2009) “Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling.” Nat Biotechnol. 27(3): 275-280.
[2] Li W, Sun W, Zhang Y, Wei W, Ambasudhan R, Xia P, Talantova M, Lin T, Kim J, Wang X, Kim W, Lipton SA, Zhang K, Ding S. (2011) “Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors.” PNAS. 108(20): 8299-8304.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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