预优化DNA转染试剂-CV-1济南思科生物科技有限公司

性能参数

产品名称: 预优化DNA转染试剂-CV-1
英文名称: GenJet™ In Vitro DNA Tranfection Reagent for CV-1 Cells
产品货号: SL100489-CV1
产品规格: 毫升
产品产地: 美国
产品商标: SignaGen
价    格: 1560元
保存与运输: 4°C
现货状态: 现货
产品资料: 实验方法技术资料
更新时间: 2019/7/12 13:46:00
浏览人数:168
诚信指数:164点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

供应商联系卡

济南思科生物科技有限公司
地址:
济南市经六路689号绿地·玫瑰坊A座734号
邮编:
250021
电话:
0531-88807138 85912162
传真:
0531-85912162
联系人:
李先生
所在区域:
山东·济南
邮件:
公司展台:

产品详细描述

Description

GenJet™ DNA In Vitro Tranfection Reagent for CV-1 is pre-optimized for transfecting CV-1 cells. The CV-1 cell line was initiated in March of 1964 with a tissue section excised from the kidney of an adult male African green monkey (Cercopithecus aethiops). The fibroblast line was originally utilized in research focusing on the transformation of the cancer-causing Rous sarcoma virus (RSV), but now is popular as a host for acquired immunodeficiency disease (AIDS) research, as well as transfection experiments with simian virus 40 (SV40) and recombinant plasmid vectors. CV-1 cells exhibit fibroblast morphology, grow adherently to glass or plastic surfaces, and are negative for reverse transcriptase. The cells are known to be susceptible to several viruses, including poliovirus 1, herpes simplex, simian virus 40, California encephalitis, and both Eastern and Western equine encephalitis. The cells exhibit rapid growth and chromosome number shifts have been reported to occur at high passage levels.

Refer to the following optimal transfection conditions for maximal transfection efficiency on CV-1 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
No
36 hours


EGFP
pEGFP-N3 (CMV promoter) 

75%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on CV-1 Cells. CV-1 cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection 

Data Sheet.pdf

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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