早幼粒细胞白血病蛋白(PML)重组蛋白

YB221Hu012
Promyelocytic Promyelocytic Promyelocytic Promyelocytic Leukemia Leukemia Leukemia Leukemia Protein Protein Protein Protein (PML)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) 早幼粒细胞白血病蛋白(PML)重组蛋白Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
4th Edition (Revised in August, 2012)
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Promyelocytic Leukemia Protein
Synonyms: Synonyms: Synonyms: Synonyms: PML, MYL, RNF71, TRIM19
Species: Species: Species: Species: Human
Size: 1mg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus; nucleoplasm; 早幼粒细胞白血病蛋白(PML)重组蛋白Cytoplasm; Endoplasmic reticulum

membrane; Peripheral membrane protein; Cytoplasmic side; Early endosome
membrane.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Gln59~Glu239 (Accession # P29590), with two N-terminal Tags, His-tag and
S-tag.
Grade & Purity: Purity: Purity: Purity: >92%, 26 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl, 5% sucrose.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 26.2 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.9
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
早幼粒细胞白血病蛋白(PML)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-QC QAEAKCPKLL PCLHTLCSGC
LEASGMQCPI CQAPWPLGAD TPALDNVFFE SLQRRLSVYR QIVDAQAVCT RCKESADFWC FECEQLLCAK CFEAHQWFLK
HEARPLAELR NQSVREFLDG TRKTNNIFCS NPNHRTPTLT SIYCRGCSKP LCCSCALLDS SHSELKCDIS AEIQQRQEE
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Shen T.H., et al. (2006) Mol. Cell 24:331-339.
2. Gresko E., et al. (2009) Oncogene 28:698-708.
3. Cuchet D., et al. (2011) J. Cell Sci. 124:280-291.
4. Jensen K., et al. (2001) Oncogene 20:7223-7233.