胎球蛋白B(FETUB)重组蛋白-蛋白质/抗原/多肽-试剂-生物在线
胎球蛋白B(FETUB)重组蛋白

胎球蛋白B(FETUB)重组蛋白

商家询价

产品名称: 胎球蛋白B(FETUB)重组蛋白

英文名称: Recombinant Fetuin B (FETUB)

产品编号: YB860Ra021

产品价格: 0

产品产地: 中国/美国

品牌商标: 钰博生物/Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: null

上海钰博生物科技有限公司
  • 联系人 : 陈环环
  • 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 183****2235
  • 传真 : 021-60514606
  • 邮箱 : shybio@126.com

 胎球蛋白B(FETUB)重组蛋白

YB860Ra021
FetuinB (FETUB)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
胎球蛋白B(FETUB)重组蛋白[ PROPERTIES ]
Residues: Met149~Asp255 (Accession # Q9UGM5),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 43.7kDa
胎球蛋白B(FETUB)重组蛋白Accurate Molecular Mass: 48kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
胎球蛋白B(FETUB)重组蛋白Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFE RQHM DSP DLGTLEV LFQGP LGSEF -MT CPDCPSSIPT
DSSNHQVLEA ATESLAKYNN ENTSKQYSLF KVTRASSQWV VGPSYFVEYL IKESPCTKSQ
ASSCSLQSSD SVPVGLCKGS LTRTHWEKFV SVTCD
[ REFERENCES ]
1. Olivier E., et al. (2000) Biochem. J. 350:589-597. 2. Hsu S.J., et al. (2004) Genome 47:931-946. 3. Dietzel E., et al. (2013) Dev. Cell 25:106-112. 4. Wajih N., et al. (2004) J. Biol. Chem. 279:43052-43060.