髓过氧化物酶(MPO)重组蛋白

YB601Ra011
Recombinant Myelin Associated Glycoprotein (MAG)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 髓过氧化物酶(MPO)重组蛋白[ PROPERTIES ]
Residues: Ser133~Lys237
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P20916
Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 髓过氧化物酶(MPO)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 43.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 髓过氧化物酶(MPO)重组蛋白hich was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
SVLDIVNT PNIVVPPEVV AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPAVLGRLR
EDEGTWVQVS LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVK
[ REFERENCES ]
1. Sato S., et al. (1989) Biochem. Biophys. Res. Commun. 163:1473-1480. 2. Spagnol G., et al. (1989) J. Neurosci. Res. 24:137-142. 3. Burger D., et al. (1993) Biochem. Biophys. Res. Commun. 197:457-464. 4. DAlfonso S., et al. (2002) J. Neuroimmunol. 126:196-204.