磷脂酶B(PLB)重组蛋白

YB048Hu013
Recombinant Phospholipase B (PLB)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 磷脂酶B(PLB)重组蛋白[ PROPERTIES ]
Residues: Met1036~Leu1458
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q6P1J6
Host: E. coli
Subcellular Location: Apical cell membrane,
Single-pass type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 磷脂酶B(PLB)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 51.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 磷脂酶B(PLB)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
MPITC PTQNEPFLRT PRNSNYTYPI KPAIENWGSD FLCTEWKASN SVPTSVHQLR
PADIKVVAAL GDSLTTAVGA RPNNSSDLPT SWRGLSWSIG GDGNLETHTT LPNILKKFNP
YLLGFSTSTW EGTAGLNVAA EGARARDMPA QAWDLVERMK NSPDINLEKD WKLVTLFIGV
NDLCHYCENP EAHLATEYVQ HIQQALDILS EELPRAFVNV VEVMELASLY QGQGGKCAML
AAQNNCTCLR HSQSSLEKQE LKKVNWNLQHGISSFSYWHQ YTQREDFAVV VQPFFQNTLT
PLNERGDTDL TFFSEDCFHF SDRGHAEMAI ALWNNMLEPV GRKTTSNNFT HSRAKLKCPS
PESPYLYTLR NSRLLPDQAE EAPEVLYWAV PVAAGVGLVV GIIGTVVWRC RRGGRREDPP
MSLRTVAL