角蛋白28(KRT28)重组蛋白

YB214Mu014
Recombinant Keratin 28 (KRT28)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[角蛋白28(KRT28)重组蛋白 PROPERTIES ]

Residues: Met1~Phe462
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: A6BLY7
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
角蛋白28(KRT28)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.2
Predicted Molecular Mass: 80.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
角蛋白28(KRT28)重组蛋白MS L RFS GGS R HVGIQSGS L R PPSG GAG FAG SSVAG GSVAG SGF SWA LGG T
LGSAPGGSHA TGALGNVSGV CFIGSEGGLL SGNEKVTMQN LNNRLASYLD NVKALEEANS
ELERKIKTWH EKYGPGSCRG LDRDYSKYHL TIEDLKSKII SSTAANANII LQIDNARLAA
DDFRLKYENE LTLHQNVEAD INGLRRVLDE LTLCRTDQEL QYESLSEEMT YLKKNHEEEM
KV LQ CA AG GN VNV EM NA APG VD LTV L L N NM RAEY EA LA EQ NRR DA EAW FQ
EKSATLQQQI SNDLGAATSA RTELTELKRS LQTLEIELQS LSATKHSLEC SLAETEGNYC
SQLAQIQAQI SALEEQLHQV RTETEGQKLE HEQLLDIKAH LEKEIETYCR LIDGDENSCS
VSKGFESGTS GNSPKDVSKT TLVKTVVEEI DQRGKVLSSR IHSIEEKMSK MSNGKAEQRV
PF