骨粘连蛋白(ON)重组蛋白

YB791Rb011
Osteonectin Osteonectin Osteonectin Osteonectin (ON)
Organism: Organism: Organism: Organism: Mus musculus musculus musculus musculus (Mouse) (Mouse) (Mouse) (Mouse)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
骨粘连蛋白(ON)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Osteonectin
Synonyms: Synonyms: Synonyms: Synonyms: ON
Size: 100 µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted, extracellular space, extracellular matrix, basement
membrane.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Ala18~Ile302 (Accession # P07214), with a N-terminal his-tag.
骨粘连蛋白(ON)重组蛋白Grade & Purity: Purity: Purity: Purity: >95%, 33.8 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 33.8 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 4.9
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS, PH7.2-PH7.4.
骨粘连蛋白(ON)重组蛋白[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months .
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a N-terminal his-tag. Their sequence is listed below.
MGHHHHHHSGS-APQ QTEVAEEIVE EETVVEETGV PVGANPVQVE MGEFEDGAEE TVEEVVADNP CQNHHCKHGK
VCELDESNTP MCVCQDPTSC PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY IAPCLDSELT
EFPLRMRDWLKNVLVTLYER DEGNNLLTEK QKLRVKKIHE NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH
PIDGYLSHTE LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL VI
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Villarreal XC., et al. (1989) Biochemistry 28 (15): 6483–91.
2. Guweidhi A., et al. (2005) Ann. Surg. 242 (2): 224–234.
3. Wasi S., et al. (1984) Can. J. Biochem. Cell Biol. 62 (6): 470–8.
4. Young MF., et al. (1992) Clin. Orthop. Relat. Res. (281): 275–94.
5. Lane TF., et al. (1994) FASEB J. 8 (2): 163–73.