蛋白二硫化物异构酶A5(PDIA5)重组蛋白

YB775Hu013
Recombinant Protein Disulfide Isomerase A5 (PDIA5)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 蛋白二硫化物异构酶A5(PDIA5)重组蛋白[ PROPERTIES ]
Residues: Trp378~Glu506
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q14554
Host: E. coli
Subcellular Location: Endoplasmic reticulum
lumen.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 蛋白二硫化物异构酶A5(PDIA5)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.2
Predicted Molecular Mass: 18.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 蛋白二硫化物异构酶A5(PDIA5)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
WMQ NPEAPPPPEP TWEEQQTSVL HLVGDNFRET LKKKKHTLVM FYAPWCPHCK
KVIPHFTATA DAFKDDRKIA CAAVDCVKDK NQDLCQQEAV KGYPTFHYYH YGKFAEKYDS
DRTELGFTNY IRALRE