分子量:42.7 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Serpin E2, His Tag by the LAL method.

Buffer:20 mM NaAc, 100 mM NaCl,  PH6.5

生物活性:Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2.

产品特性:Human Serpin E2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 42.7 kDa. The predicted N-terminus is Ser 20. The reducing (R) protein migrates as 45-48 kDa in SDS-PAGE  due to glycosylation.

产品背景:SERPINE2 is also known as Glia-derived nexin (GDN), Peptidase inhibitor 7 (PI7), Protease nexin 1(PN1). SERPINE2 is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). PAI-1 / GDN  is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis. Defects in Serpin E1 / PN1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma.