分子量:30.4 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Carbonic Anhydrase III, His Tag by the LAL method.

Buffer:50 mM Tris, 150 mM NaCl,  pH 7.5

生物活性:Measured by its esterase activity. The specific activity is >1 pmol/min/g.

产品特性:Human Carbonic Anhydrase III, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 30.4 kDa. The predicted N-terminus is Met 1. The reducing (R) protein migrates as 28-30 kDa in SDS-PAGE .

产品背景:Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.