分子量:38.7 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human BTLA (31-134), Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by its binding ability in a functional ELISA.
Immobilized Human HVEM, Strep Tag (Cat # HVM-H5283) at 2 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag (Cat # BTA-H5255) with a linear range of 0.01  0.5 μg/mL.

产品特性:Human BTLA (31-134), Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 38.7 kDa. The predicted N-terminus is Lys 31. The reducing (R) protein migrates as 45-50 kDa in SDS-PAGE  due to glycosylation.

产品背景:B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.