分子量:52.9 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Siglec-3, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. TheED50 forthiseffectistypically1.2-4.8g/mL.

产品特性:Human Siglec-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 52.9 kDa. The predicted N-terminus is Asp 18. The reducing (R) protein migrates as 64-85 kDa in SDS-PAGE  due to glycosylation.

产品背景:Myeloidcellsurfaceantigen CD33 is also known as SIGLEC3, Siglecs(sialicacidbindingIglikelectins) andGP67, is a single-pass type I membrane protein which belongs to theimmunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.