Arf6ProteinΔ12Q67Lmutant-蛋白质/抗原/多肽-试剂-生物在线
武汉纽斯特生物技术有限公司
Arf6ProteinΔ12Q67Lmutant

Arf6ProteinΔ12Q67Lmutant

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产品名称： Arf6ProteinΔ12Q67Lmutant

英文名称： Arf6Protein Δ12 Q67L mutant

产品编号： 10125

产品价格： 0

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

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Arf6 Protein Δ12 Q67L mutant [10125]
[10 µg]		  
Catalog Number:  10125
Product Name:  Arf6 Protein Δ12 Q67L mutant
Synonyms:  ADP-ribosylation factor 6
Source:    Human, recombinant, His6-tag
Expression Host:   E. coli
Molecular Weight:   20 kDa
Purity:    >95% by SDS-PAGE
Introduction:   Arf6 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and the actin cytoskeketon at the plasma membrane and functions as a regulatory molecule of phagocytosis.
Amino Acid Sequence   (1-175, Δ12, Q67L)
MGKVLSKIFGN-EMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGL 
DKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHE 
IQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  1mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf6 Δ12 Q67L mutant was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
References:  
1. Cavenagh, M. M. et al., J. Biol. Chem. 271: 21767-21774, 1996. 
2. DSouza-Schorey, C. et al., Science 267: 1175-1178, 1995. 
3. Falace, A. et al., Am. J. Hum. Genet. 87: 365-370, 2010. 
4. Hernandez-Deviez, D. J. et al., Nature Neurosci. 5: 623-624, 2002. 
5. ONeal, C. J. et al., Science 309: 1093-1096, 2005.