DNA纯化与浓缩™-500 (DCC™-500)-核酸纯化-试剂-生物在线
DNA纯化与浓缩™-500 (DCC™-500)

DNA纯化与浓缩™-500 (DCC™-500)

商家询价

产品名称： DNA纯化与浓缩™-500 (DCC™-500)

英文名称： DNA Clean & Concentrator™-500

产品编号： D4031

产品价格： 0

产品产地： 美国

品牌商标： ZYMO RESEARCH

更新时间： null

使用范围： null

北京天漠科技开发有限公司
  • 联系人 :
  • 地址 : 北京市朝阳区北苑路170号凯旋城E座1809室
  • 邮编 : 100101
  • 所在区域 : 北京
  • 电话 : +86-10-139****7531
  • 传真 : +86-10-58235289
  • 邮箱 : fjan@zymoresearch.com.cn
进入展台

 HIGHLIGHTS

•
Simple, rapid recovery of ultra-pure DNA from large-scale sample sources.
• Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. Unique column construction allows sample washing to be performed using a centrifuge or vacuum source.
• Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.


SPECIFICATIONS 
• Format: Spin Column.
• DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
• DNA Size Limits: From 50 bp to 23 kb.
• DNA Recovery: Typically, ≤ 500 µg total DNA can be eluted with ≥ 1 ml water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.
• Sample Sources: DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates.
• Product Detergent Tolerance: ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS.
• Equipment: Microcentrifuge and centrifuge, vacuum source, or syringe.



DESCRIPTION

The DNA Clean & Concentrator™-500 was designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples including large-scale restriction endonuclease digestions and "crude" DNA preparations. The DCC™-500 features a single buffer system that allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ column. The DNA is washed twice using a centrifuge or vacuum source then eluted with a small volume (≥ 1 ml) of water using a centrifuge. The procedure is easy: simply add the specially formulated DNA Binding Buffer to your sample then transfer the mixture to the supplied to your sample then transfer the mixture to the supplied Zymo-Spin™ column. There is no need for organic denaturants or chloroform. Instead, the product features unique column technology to yield high quality, purified DNA in just minutes. DNA purified using the DCC™-500 is suitable for DNA sequencing, DNA transfection, RNA transcription, microarray analysis, PCR, nucleotide blotting, restriction endonuclease digestion, etc.
Loading and washing the Zymo-Spin VI™ Column can be performed using any combination of the following: vacuum or centrifugation.



ORDERING INFORMATION:
Products Description
Protocol
Cat No.
Size

   Price

  (RMB)

  
DNA Clean &
Concentrator™-500
 For 10 DNA preps.
D4031
1 kit
  
 
For 20 DNA preps.
D4032
1 kit