The OneStep™ and OneStep-96™ PCR Inhibitor Removal Kits contain all the components necessary to efficiently remove contaminants from DNA and RNA preparations that can inhibit downstream enzymatic reactions such as PCR and RT, respectively. The column/plate matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. Polyphenolic compounds can contaminate impure DNA and RNA preparations from soil, plants, skin, and fecal samples amongst others. Sample cleanup is as simple as: applying, spinning and recovering a sample from the column or plate.

DNA is efficiently amplified by PCR following humic acid removal with the OneStep™ PCR Inhibitor Removal Kit. The figure shows amplification of a 200 bp product from DNA containing humic acid that was “treated” with the kit. Alternatively, PCR amplification was completely inhibited in the case of the “non-treated” sample. In each case, equal amounts of DNA were used for each PCR and equivalent amounts of the reaction were then analyzed in a 2.0% (w/v) agarose/TAE/EtBr gel. The ladder is a 100 bp DNA marker (Zymo Research). Hot start PCR was performed using ZymoTaq™ PreMix (Zymo Research).