Human Methylated & Non-methylated DNA Set北京天漠科技开发有限公司

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产品名称: Human Methylated & Non-methylated DNA Set
英文名称: Human Methylated & Non-methylated DNA Set
产品货号: D5014
产品规格: 1 set
产品产地: 美国
产品商标: ZYMO RESEARCH
价    格: 询价
保存与运输: 常温  快递
现货状态: 期货
产品资料: 实验方法技术资料
更新时间: 2015/12/30 11:36:00
浏览人数:1596
诚信指数:274点
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 
Human Methylated & Non-methylated DNA Set
 
Purified, non-methylated and methylated human DNA (5 µg/20 µl of each) for use as negative and positive control in methylation detection applications.
Set of control primers designed to amplify non-methylated, methylated, and mixed methylation copies of the death-associated protein kinase 1 gene (DAPK1) following bisulfite conversion.
Can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, and EZ DNA Methylation-Direct kits to assess the efficiency of bisulfite-mediated conversion of DNA.
 

The Human HCT116 DKO Non-methylated DNA is purified from cells that contain genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-)1. The DNA derived from HCT116 DKO cells has a low level of DNA methylation (< 5%) and can be used as a negative control for DNA methylation analysis (Figure). The Human HCT116 DKO Methylated DNA is purified HCT116 DKO DNA that has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2 (EC 2.1.1.37;) and can be used as a positive control for DNA methylation analysis.

An assay for complete methylation by M.SssI methytransferase. Digestion of non-methylated and methylated HCT116 DKO DNA with restriction enzymes MspI and HpaII. MspI digests both nonmethylated and methylated DNA. HpaII is sensitive to CpG methylation.

Methylated cytosines comprising CG dinucleotides within DNA remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted to uracil and detected as thymine following PCR. The control primers, DAPK1 primer I and DAPK1 primer II amplify methylated, non-methylated, and mixed methylation copies of the death-associated protein kinase 1 gene and are intended for use after bisulfite conversion of the control

ORDERING INFORMATION

Product                                             Catalog No.     Size     Price(RMB)

Human Methylated & Non-methylated DNA Set    D5014        1 set   

 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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