17544202
            
FICOLL PAQUE PREMIUM 6X100ML
            
价格优惠 
        
        

            
17544203
            
FICOLL PAQUE PREMIUM 6X500ML
            
价格优惠 
        
        
    

应用范围：从外周血，骨髓及脐带血分离人单个核细胞
密度：1.077g/ml

Ficoll-Paque PREMIUM products are available in densities of 1.073, 1.077, and 1.084 g/ml. 
Ficoll-Paque™ PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque™ PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured in a strictly controlled environment compliant with ISO 13485:2003 and in accordance with GMP1 and the recommendations of the United States Pharmacopeia2 for the manufacture of cell therapy products. 

    

        

            

            
ECHNICAL SPECIFICATIONS
            
        
        

            

            
Properties

            Ficoll-Paque™ PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow.  
            
        
        

            

            
Density

            Ficoll-Paque PREMIUM:

            Ficoll-Paque PREMIUM 1.084:

            Ficoll-Paque PREMIUM 1.073: 
            
            

            


            1.077 ±0.001 g/ml

            1.084 ±0.001 g/ml

            1.073 ±0.001 g/ml 
            
        
        

            

            
Stability

            Ficoll-Paque™ PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque™ PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution.  
            
        
        

            

            
Endotoxins

            Contains < 0.12 EU/ml 
            
        
        

            

            
Typical results from our laboratories using Ficoll-Paque™ PREMIUM  (density 1.077 g/ml) and fresh peripheral blood (< 2 h old)
            
        
        

            

            
Mononuclear cells
            

                
60 ±20 recovery of mononuclear cells from the original blood sample
                
> 90 of cells present in the final preparation are mononuclear cells
                
> 90 viability (measured by trypan blue exclusion)
            
            
 
            
        
        

            

            
Other cells
            

                
Maximum 5 granulocytes
                
Maximum 10 erythrocytes
            
            
 
            
        
    

Applications
Ficoll-Paque PREMIUM

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood. 
Ficoll-Paque PREMIUM 1.084

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats. 
Ficoll-Paque PREMIUM 1.073

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes). 
References 

    
EC Guide to GMP (Good Manufacturing Practice) annex 1 "Manufacture of Sterile Medicinal Products"
    
United States Pharmacopeia. Recommendations for ancillary materials, Chapter <1043>.