Reagent
PEIpro®
Concentration
1 mg/mL
Molecule delivered
Plasmid DNA and co-delivery of several plasmid DNA
Applications
Large-scale virus production
Virus types
Mainly adenovirus, Adeno-Associated Viruses (AAV), Lentivirus, influenza virus, retrovirus, Virus-like particles (VLP)
Cell types
Mammalian producer cell lines (HEK-293, HEK-293 derivatives, BHK, VERO cell lines, virus-specific packaging cell lines.
Number of transfections
100 mL of PEIpro® transfection reagent is sufficient to transfect on average 50 L of cell culture
Storage
Store PEIpro® at 5 °C ± 3°C.
Provided with
Protocol
Certificate of Analysis
Certificate of Origin
Non-Hazardous Product Statement
Summary
PEIpro® transfection reagent is the leading chemical-based DNA transfection reagent that offers flexibility and scalability to develop a robust and sustainable Process Development for viral vector production. PEIpro® benefits from extensive research development in PEI polymer chemistry and formulation to achieve highest transfection efficiency in both adherent and suspension cell culture systems. Protocols established with PEIpro® during Process Development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of PEIpro®: PEIpro®-HQ and PEIpro®-GMP.