英文版：Nuclear and Cytoplasmic Extraction Kit

 Catalog No: KC-435

Size: 1 Kit

I. Introduction

Nuclear and Cytoplasmic Extraction Kits provides a complete system that enable stepwise separation and preparation of cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmid fractions quickly with little or no cross-contaminations. Nuclear extracts obtained with the NE-PER® Nuclear and Cytoplasmic Extraction Reagents are compatible with a variety of downstream applications including Western blotting, the BCA Protein Assay, gel shift assays, reporter gene assays and enzyme activity assays.

II. Kit Contents:

A. Compoments (100 assays)

•   Cytosol Extraction Reagent A (CER-A)                20 ml

•   Cytosol Extraction Reagent B (CER-B)               1.2 ml

•   Nuclear Extraction Reagent (NER)                      10 ml

•   DTT (1 M)                                                                  100 μl

•   Protease Inhibitor Cocktail                                    500 μl

•   PMSF                                                                         500 μl

•   Phosphotase Inhibitor Cocktail                            500 μl

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