Description

Smooth muscle is responsible for the contractility of hollow organs, such as blood vessels, the gastrointestinal tract, the bladder, and the uterus. Its structure differs greatly from that of skeletal muscle. The human stomach contains three layers of muscle in its walls, the outer longitudinal, the middle circular and the inner oblique and visceral smooth muscle cells makes up all three layers along the entire organ. Smooth muscle contraction is critical to peristalsis in the human stomach and the contraction may be mediated by activation of phospholipase through two distinct mechanisms (increased intracellular Ca2+ and G protein activation) and activating PKCepsilon-dependent mechanisms [1]. In vitro study also shows that gastric smooth muscle cells express ET and eNOS [2, 3] and both calcium and sodium may be involved as current carriers in the generation of the plateau potential.

HGSMC from ScienCell Research Laboratories are isolated from the human stomach. HGSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HGSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HGSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGSMC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use smooth muscle cell medium (SMCM, Cat. No. 1101) for the culturing of HGSMC in vitro.

Product Use

HGSMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Cao, W., Chen, Q., Sohn, U. D., Kim, N., Kirber, M. T., Harnett, K. M., Behar, J., Biancani, P. (2001) Ca2+-induced contraction of cat esophageal circular smooth muscle cells. Am J Physiol Cell Physiol. 280(4):C980-92.
[2] Kitsukawa, Y., Gu, Z. F., Hildebrand, P. and Jensen, R. T. (1994) Gastric smooth muscle cells possess two classes of endothelin receptors but only one alters contraction. Am J Physiol Gastrointest Liver Physiol 266: G713-G721.
[3] Teng, B.-Q., Murthy, K. S., Kuemmerle, J. F., Grider, J. R., Sase, K., Michel, T., and Makhoouf, G. M. (1998) Expression of endothelial nitric oxide synthase in human and rabbit gastrointestinal smooth muscle cells. Am J Physiol Gastrointest Liver Physiol 275: G342-G351.