Recombinant Human TNF-α

Catalog Number:

300-01A

Synonyms:

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor （DIF）, Necrosin, Cytotoxin

Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein （17.4 kDa） which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

AA Sequence:

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Source:

E.coli

Purity:

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity:

The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

Cross Reactivity:

Cow, Monkey, Mouse, Rat, Sheep, Human, Bacteria, Human + Rat, Pig

Research Interests:

	AIDS / HIV		Angiogenesis/ Cardiovascular
	Apoptosis		Cancer
	Diabetes / Weight Regulation		Immune System
	Inflammation		Neurobiology
	Stem Cells & Differentiation		TNF Superfamily
	Allergy		Transplantation

Country of Origin:

USA

Research Use Only. Not for use in diagnostic or therapeutic procedures.