Disease:

 Anaplastic astrocytoma grade III 

Origin:

Derived from left malignant glioma 

Species:

Homo sapiens 

Tissue:

Brain 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Tumorigenic 

Patient:

Male, Caucasian, 69 yrs of age 

Medium:

DMEM + 10% FBS 

Subculture:

1:2 to 1.4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 Not known 
DNA Profile (STR):
 Not known 
Related cells by Organ:

B103 

LN-319 

SH-Sy5y cells 

U87MG cells 


References for LN-319 cells:

1. Grouzmann, E., Meyer, C., Burki, E., and Brunner, H. Neuropeptide Y Y2 receptor signalling mechanisms in the human glioblastoma cell line LN319. Peptides, 22: 379-386, 2001. 

2. Kato, Y., Kaneko, M. K., Kuno, A., Uchiyama, N., Amano, K., Chiba, Y., Hasegawa, Y., Hirabayashi, J., Narimatsu, H., Mishima, K., and Osawa, M. Inhibition of tumor cell-induced platelet aggregation using a novel anti-podoplanin antibody reacting with its platelet-aggregation-stimulating domain. Biochem Biophys Res Commun, 349: 1301-1307, 2006. 

3. Kato, Y., Vaidyanathan, G., Kaneko, M. K., Mishima, K., Srivastava, N., Chandramohan, V., Pegram, C., Keir, S. T., Kuan, C. T., Bigner, D. D., and Zalutsky, M. R. Evaluation of anti-podoplanin rat monoclonal antibody NZ-1 for targeting malignant gliomas. Nucl Med Biol, 37: 785-794. 

4. Meager, A., Visvalingam, K., Dilger, P., Bryan, D., and Wadhwa, M. Biological activity of interleukins-28 and -29: comparison with type I interferons. Cytokine, 31: 109-118, 2005. 

5. Sivasankaran, B., Degen, M., Ghaffari, A., Hegi, M. E., Hamou, M. F., Ionescu, M. C., Zweifel, C., Tolnay, M., Wasner, M., Mergenthaler, S., Miserez, A. R., Kiss, R., Lino, M. M., Merlo, A., Chiquet-Ehrismann, R., and Boulay, J. L. Tenascin-C is a novel RBPJkappa-induced target gene for Notch signaling in gliomas. Cancer Res, 69: 458-465, 2009.