Disease:

Hepatocellular carcinoma 

Origin:

Established from the tumor tissue of an 8-year-old black boy 

Species:

Homo sapiens 

Tissue:

Liver 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Human hypotriploid stemline with near tetraploid side line 

Patient:

Male, 8 years of age 

Medium:

MEM + 10% FBS 

Subculture:

1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 II 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins 
DNA Profile (STR):
 Amelogenin: X; CSF1PO: 8; D13S317: 12,14; D16S539: 10; D5S818: 13; D7S820: 8,10; THO1: 6,7; TPOX: 9; vWA: 17 
Related cells by Organ:

Hep3B cells 

HepG2 cells 


References for Hep3B cells:

1. Wang, J., Wang, X. F., Zhang, L. G., Xie, S. Y., Li, Z. L., Li, Y. J., Li, H. H., and Jiao, F. Involvement of the mitochondrial pathway in p53-independent apoptosis induced by p28GANK knockdown in Hep3B cells. Cytogenet Genome Res, 125: 87-97, 2009. 

2. Ji, L., Shen, K., Liu, J., Chen, Y., Liu, T., and Wang, Z. Intracellular glutathione regulates Andrographolide-induced cytotoxicity on hepatoma Hep3B cells. Redox Rep, 14: 176-184, 2009. 

3. Yildirim, H. and Kockar, F. TGF-beta upregulates tumor-associated carbonic anhydrase IX gene expression in Hep3B cells. Cell Biol Int, 33: 1002-1007, 2009. 

4. Jin, C. Y., Park, C., Kim, G. Y., Lee, S. J., Kim, W. J., and Choi, Y. H. Genistein enhances TRAIL-induced apoptosis through inhibition of p38 MAPK signaling in human hepatocellular carcinoma Hep3B cells. Chem Biol Interact, 180: 143-150, 2009. 

5. Zimonjic, D. B., Zhou, X., Lee, J. S., Ullmannova-Benson, V., Tripathi, V., Thorgeirsson, S. S., and Popescu, N. C. Acquired genetic and functional alterations associated with transforming growth factor beta type I resistance in Hep3B human hepatocellular carcinoma cell line. J Cell Mol Med, 13: 3985-3992, 2009. 

6. Seo, S. H., Shin, D. Y., Hong, S. H., Park, S. E., Choi, Y. H., and Hong, S. H. Euphorbiae humifusae induces apoptosis in Hep3B hepatocarcinoma cells via simultaneous activation of the death receptor-mediated and mitochondrial pathways. Mol Med Report, 2: 221-227, 2009.