Disease:

 Prostatic adenocarcinoma grade IV 

Origin:

 Derived from metastatic bone 

Species:

 Homo sapiens 

Tissue:

 Prostate 

Morphology:

 Epithelial 

Properties:

 Adherent 

Cytogenic data:

 The line is near-triploid 

Patient:

 Male, Caucasian, 62 yrs of age 

Medium:

 RPMI1640 + 10% FBS 

Subculture:

 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

 Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 HLA A1, A9 
DNA Profile (STR):
 Amelogenin: X CSF1PO: 11; D13S317: 11; D16S539: 11; D5S818: 13; D7S820: 8,11; THO1: 6,7; TPOX: 8,9; vWA: 17 
Mutations in PC-3 Cells 

Related cells by Organ:

PC-3 cells 

VcaP cells 


References for PC-3 cells:

1. Vyas, H. K., Pal, R., Lohiya, N. K., and Talwar, G. P. A partner monoclonal antibody to Moab 730 kills 100% of DU145 and PC3 androgen-independent cancer cells. J Biosci, 34: 909-916, 2009. 

2. Roca, H., Varsos, Z. S., and Pienta, K. J. CCL2 is a negative regulator of AMP-activated protein kinase to sustain mTOR complex-1 activation, survivin expression, and cell survival in human prostate cancer PC3 cells. Neoplasia, 11: 1309-1317, 2009. 

3. Olea-Herrero, N., Vara, D., Malagarie-Cazenave, S., and Diaz-Laviada, I. The cannabinoid R+ methanandamide induces IL-6 secretion by prostate cancer PC3 cells. J Immunotoxicol, 6: 249-256, 2009. 

4. Pei, Z., Li, H., Guo, Y., Jin, Y., and Lin, D. Sodium selenite inhibits the expression of VEGF, TGFbeta(1) and IL-6 induced by LPS in human PC3 cells via TLR4-NF-(K)B signaling blockage. Int Immunopharmacol, 10: 50-56. 

5. Olea-Herrero, N., Vara, D., Malagarie-Cazenave, S., and Diaz-Laviada, I. The cannabinoid R(+)methanandamide induces IL-6 secretion by prostate cancer PC3 cells. J Immunotoxicol, 2009. 

6. Chen, J., Xia, D., Luo, J. D., and Wang, P. Exogenous p27KIP1 expression induces anti-tumour effects and inhibits the EGFR/PI3K/Akt signalling pathway in PC3 cells. Asian J Androl, 11: 669-677, 2009. 

7. Zhou, Q., Yan, B., Hu, X., Li, X. B., Zhang, J., and Fang, J. Luteolin inhibits invasion of prostate cancer PC3 cells through E-cadherin. Mol Cancer Ther, 8: 1684-1691, 2009.