Disease:

 Endometrial Carcinoma G2 

Origin:

 Derived from endometrial carcinoma 

Species:

 Homo sapiens 

Tissue:

 Uterus 

Morphology:

 Epithelial 

Properties:

 Adherent 

Cytogenic data:

 Tumorigenic 

Patient:

 Female, Asian 

Medium:

 IMDM + 10% FBS 

Subculture:

 1:2 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

 Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 G6PD(typeB), NP, LDH 
DNA Profile (STR):
 D5S818:10,11,12; D13S317:9,10,11; D7S820:10,11,14; D16S539:11,12,13; VWA:17,20,22; TH01:7,9.3; Amelogenin:X; TPOX:11,14; CSF1PO:9,10 
Mutations in HEC-59 Cells 

Related cells by Organ:

HEC-59 cells 

HeLa cells 

SiHa cells 

CaSKi cells 

C33A cells 


References for HEC-59 cells:

1. Hiramatsu, H. P., Kikuchi, Y., Seto, H., and Nagata, I. In vitro sensitivity of human endometrial cancer cell lines HEC-59 to paclitaxel or irinotecan (CPT-11) in combination with other aniticancer drugs. Anticancer Drugs, 11: 573-578, 2000. 

2. Suzuki, T., Sasano, H., Sasaki, H., Fukaya, T., and Nagura, H. Quantitation of P450 aromatase immunoreactivity in human ovary HEC-59 during the menstrual cycle: relationship between the enzyme activity and immunointensity. J Histochem Cytochem, 42: 1565-1573, 1994. 

3. Sasaki, H. [Aromatase activities of endometrial carcinomas HEC-59 and both basic and clinical analyses of endometrial hyperplasia as a premalignant disease]. Nihon Sanka Fujinka Gakkai Zasshi, 45: 763-775, 1993. 

4. Tada, A., Sasaki, H., Nakamura, J., Yoshihama, M., and Terashima, Y. Aromatase activity and the effect of estradiol and testosterone on DNA synthesis in endometrial carcinoma cell lines HEC-59. J Steroid Biochem Mol Biol, 44: 661-666, 1993. 

5. Yamamoto, T., Kitawaki, J., Urabe, M., Honjo, H., Tamura, T., Noguchi, T., Okada, H., Sasaki, H., Tada, A., Terashima, Y., and et al. Estrogen productivity of endometrium and endometrial cancer tissue; influence of aromatase on proliferation of endometrial cancer cells HEC-59. J Steroid Biochem Mol Biol, 44: 463-468, 1993. 

6. Sasaki, H., Mitsunaga, S., Kitamura, T., Kishino, Y., Toyoizumi, T., Ogawa, M., Yamauchi, S., Iwata, M., Terashima, Y., and Mochizuki, S. [Radioresistant elements in adenocarcinoma of the uterus HEC-59]. Nihon Sanka Fujinka Gakkai Zasshi, 38: 1587-1594, 1986.