Origin:

 Embryonic kidney 

Species:

Homo sapiens 

Tissue:

Kidney 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

transformed with adenovirus 5 DNA 

Medium:

MEM + 2 mM Glutamine + 10% FBS 

Subculture:

 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

II 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative


References for HEK-293 cells:

1. Graham FL, et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977.