预优化siRNA转染试剂-Smooth Muscle-克隆与表达-试剂-生物在线
预优化siRNA转染试剂-Smooth Muscle

预优化siRNA转染试剂-Smooth Muscle

商家询价

产品名称: 预优化siRNA转染试剂-Smooth Muscle

英文名称: GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells

产品编号: SL100568-SMC

产品价格: 0

产品产地: 美国

品牌商标: SignaGen

更新时间: null

使用范围: null

济南思科生物科技有限公司
  • 联系人 :
  • 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
  • 邮编 : 250021
  • 所在区域 : 山东
  • 电话 : 159****9896
  • 传真 : 0531-85912162
  • 邮箱 : jnqsw@163.com; info@signagen-china.com

Description:
GenMute™ siRNA Transfection Reagent for SMC is pre-optimized for transfecting siRNA to smooth muscle cells (SMC). 

Refer to the following optimal transfection conditions for maximal silencing on SMC. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute
 Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 

Transfection Results:
Reporter Gene
Silencing Efficiency (% )


DMEM with 4.5 g/L glucose, 10% FBS

~60%
40 nM

1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
85%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf