SJK-132-20细胞

ATCC Number： CRL-1640™
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： 淋巴样
免疫类型： IgG1
运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
Designations: SJK-132-20
Depositors: D Korn
Isotype: IgG1
SJK-132-20细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
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Antigen Expression: H-2db
Comments: Mice were immunized with fraction VIII human DNA polymerase alpha.
Alpha.
Spleen cells were fused with NS-1 myeloma cells.
SJK-132-20细胞The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species.
The cells need to be in culture for 6 to 7 days before antibody production is detectable.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-177.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
References: 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. SJK-132-20细胞PubMed: 7085672