RG7细胞

生长状态： 悬浮生长
数量： 大量
细胞形态： 淋巴样
免疫类型： mouse IgG2b
ATCC Number： TIB-173™
器官来源： 脾
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
Designations: RG7/1.30
Depositors: TA Springer
Isotype: mouse IgG2b
RG7细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast

Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat IgG2a (Fc)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [58004]
Comments:RG7细胞Animals were immunized with pooled rat IgG. 

Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:RG7细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

References: 58004: Springer TA, et al. Monoclonal antibodies specific for rat IgG1, IgG2a and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma 1: 257-273, 1982. PubMed: 6208128