Go Je细胞

ATCC Number： CRL-1381™
相关疾病： 其他疾病
是否是肿瘤细胞： 0
物种来源： 人
数量： 大量
生长状态： 贴壁生长
器官来源： 皮肤
运输方式： 冻存运输
细胞形态： 成纤维样
Designations: Go Je
Go Je细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast

Source: Organ: skin
Disease: Ehlers-Danlos syndrome (variant type)
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Gender: female
Comments: The tissue donor suffered from a variant form of Ehlers-Danlos syndrome.
Propagation:Go Je细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Go Je细胞Subculture every 6 to 8 days.