Ramos细胞

数量： 大量
运输方式： 冻存运输
生长状态： 悬浮生长
细胞形态： 淋巴样
免疫类型： IgM; lambda light chain
年限： 3 years
ATCC Number： CRL-1596™
相关疾病： 其他疾病
细胞类型： B淋巴细胞
是否是肿瘤细胞： 1
物种来源： 人
Designations: Ramos (RA 1)
Depositors: BC Giovanella
Isotype: IgM; lambda light chain
Ramos细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast

Source: Disease: Burkitts lymphoma (American)
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted) [1093] [22747]
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Applications: transfection host
Receptors: interleukin 4 (IL-4); low affinity IgE (CD23) [23468]
Tumorigenic: Yes
Antigen Expression: CD23 + [23468]
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
Ramos细胞D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Age: 3 years
Gender: male
Ethnicity: Caucasian
Comments: The cells are negative for Epstein-Barr virus.
Both the parental line (ATCC CRL-1596)and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.
The cells are reported to secrete IgM (lambda light chain).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:Ramos细胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) 

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
derivative:ATCC CRL-1923
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22747: Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343
23389: Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879
23468: Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitts lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726
26047: Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896
32796: Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. Ramos细胞PubMed: 8943082