血小板反应蛋白(THBS1)重组蛋白

YB611Ra041
Recombinant Thrombospondin 1 (THBS1)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
血小板反应蛋白(THBS1)重组蛋白[ PROPERTIES ]
Residues: Asn65~Cys270
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q28178
Host: E. coli
Subcellular Location: Endoplasmic reticulum.
Sarcoplasmic reticulum.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
血小板反应蛋白(THBS1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.5
Predicted Molecular Mass: 26.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
血小板反应蛋白(THBS1)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
NLIPPV PDKKFQDLVD AVRAEKGFLL LASLRQMKKT RGTLLAVERK DHSGQVFSVI
SNGKAGTLDL SLTVQGKQHV VSVEEALLAT GQWKSITLFV QEDRAQLYID CEKMENAELD
VPIQSIFTRD LASIARLRIA KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVFV
TLDNNVVNGS SPAIRTDYIG HKTKDLQAIC