Hs 574.T细胞

数量： 大量
生长状态： 贴壁生长
运输方式： 冻存运输
组织来源： duct
细胞形态： 成纤维样
器官来源： 乳房
是否是肿瘤细胞： 1
物种来源： 人
ATCC Number： CRL-7345™
相关疾病： 导管癌
Designations: Hs 574.T
Biosafety Level: 1
Hs 574.T细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast

Source: Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,13
D13S317: 9,12
D16S539: 9,13
Hs 574.T细胞D5S818: 11,12
D7S820: 9,11
THO1: 9,9.3
TPOX: 8,11
vWA: 16,19
Gender: female
Comments: The cells appear to be of stromal origin (connective tissue).
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing:Hs 574.T细胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended 

Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020