Arl1Protein Q71L mutant-蛋白质/抗原/多肽-试剂-生物在线
Arl1Protein Q71L mutant

Arl1Protein Q71L mutant

商家询价

产品名称: Arl1Protein Q71L mutant

英文名称: Arl1Protein Q71L mutant

产品编号: 10156

产品价格: 0

产品产地: 美国

品牌商标: NewEastBio

更新时间: null

使用范围: null

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Arl1 Protein Q71L mutant [10156]
[25 µg]
 
Catalog Number:  10156
Product Name:  Arl1 Protein Q71L mutant
Synonyms:  ADP-ribosylation factor-like 1, ARFL1
Source:   Human, recombinant full length, His6-tag
Expression Host:   E. coli
Molecular Weight:   20 kDa
Purity:   >95% by SDS-PAGE
Introduction:   Arl1 is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform.
Amino Acid Sequence   (1-181, Q71L)
MGGFFSSIFSSLFGTREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVETVTYKNLKFQVWD 
LGGLTSIRPYWRCYYSNTDAVIYVVDSCDRDRIGISKSELVAMLEEEELRKAILVVFANKQDMEQAM 
TSSEMANSLGLPALKDRKWQIFKTSATKGTGLDEAMEWLVETLKSRQ
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arl1 Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining
References:  
1. Hong, J.-X. et al., J. Biol. Chem. 273: 15872-15876, 1998. 
2. Zhang, G.-F. et al., J. Biol. Chem. 270: 21-24, 1995.