内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>98% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA against immobilized Human CD117, Fc Tag (Cat# CD7-H5255, 0.2μg/well). The linear range is 0.05-1.2 ng/mL.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5 - 8 ng/mL.


产品背景:
Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

产品描述:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation. 

SDS-PAGE