EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒杭州昊鑫生物科技股份有限公司

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产品名称: EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒
英文名称: EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒
产品货号: RN5301
产品规格: 50 次
产品产地: 国产
产品商标: 艾德莱
价    格: 1580元
保存与运输: RT
产品资料: 实验方法技术资料
更新时间: 2017/6/16 15:46:00
浏览人数:165
诚信指数:5点
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

本公司独家推出EASYspin无***酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶, 离心沉淀去除多糖多酚和次级代谢产物,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,然后RNA被选择性洗脱滤过, 吸附在基因组DNA清除柱上的残留DNA无法洗脱连同柱子一起丢弃从而清除掉DNA。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜,再通过一系列快速的漂洗-离心的步骤,去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点:

1.完全不使用***酚,氯仿等试剂,也不需要乙醇沉淀等步骤。 
2.简捷,单个样品操作一般可在25分钟内完成,世界上最简单快速的试剂盒。 
3.独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。 
4.世界第一适应性极其广泛,可以提取包括复杂中草药如石斛/丹参/雪莲/人参、复杂淀粉种子如水稻/小麦/玉米种子、复杂果实如葡萄/蓝莓/草莓/西瓜果实、复杂抗逆植物如冬青/松针/沙棘/胡杨、复杂花卉月季/玫瑰/梅/牡丹花、复杂多糖植物紫菜/仙人掌/芦荟水稻种子等数百种国内外试剂盒提取失败的样品,使用EASYspin系列植物RNA提取试剂盒累计发表文章超过100篇。详细样品列表请参考公司主页产品介绍或者找我们索取100多篇发表文章原文。 
5.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,无DNA残留,可用于荧光定量PCR、RT-PCR、芯片、二代测序、Northern-blot等各种实验。
使用EASYspin/EASYspin Plus系列植物RNA提取试剂盒已经有大量文章发表,请联系我们索取发表的文章和包括北京林业大学,北京农学院,中国农业大学,农林科学院果树研究所,林业所,中国农科院等大学和研究院所大量使用案例。

1.     桃果实、花、根、叶:Isolation, characterisation and phylogenetic analysis of resistance gene    analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science,    2011, 91(6): 961-970

2.     樱桃花、叶、颚等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium    L.)    causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December    2012

3.     洋葱根、茎、蕾、叶、雌雄蕊等各部位Cloning and Expression Analysis of A Putative B Class MADS-box    Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769

4.     芜菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid       Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January    2012,    Pages 47–59

5.        1 EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H

6.     番茄叶:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science  Bulletin 2012,28(10):132-135

7.     栀子叶:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural    Science Bulletin.2012, 28(27):194-198

8.     油桐果实:Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong.     Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding GeneVfBCCP)  from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August

9.     油桐果实1Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time    PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084

10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra    haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 3512):1814-1821

11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709

12. 石斛1ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705

13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012

14. 大豆1Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012

15. 红花玉兰:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5

16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104

17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online15 March, 2013

18. :五倍子1Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9

19. 青杄 cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401

20. 青杄 1cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206

21. 青杄 2Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49No. 10, Oct. 2013.

22. 洋葱:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y

23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17.

24. 木瓜1Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C:

25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x

26. 柑橘1Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 18.

27. 柑橘2 Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.

28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.

29. 栀子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198

30. 丹参:Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.

31. 牡丹:Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996

32. 东南景天:Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165.

33. 山苍子:Identification of appropriate reference genes for normalizing transcript expression by quantitative realtime PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1

34. 木本植物:Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0

35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.

36. 桃子:Biochemical changes and defence responses during the development of peach gummosis caused by Lasiodiplodia theobromae. Eur J Plant Pathol (2014) 138:195–207, DOI 10.1007/s10658-013-0322-4.

37. 桃子1Carbohydrate metabolism changes in Prunus persica gummosis infected with Lasiodiplodia theobromae. Phytopathology "First Look" paper • http://dx.doi.org/10.1094/PHYTO-01-13-0025-R • posted 11/27/2013.

38. 海棠:The Malus crabapple transcription factor McMYB10 regulatesanthocyanin biosynthesis during petal coloration. Scientia Horticulturae 166 (2014) 42–49.

39. 海藻:A rapid and sensitive method for field detection of Prorocentrum donghaiense using reverse transcription-coupled loop-mediated isothermal amplification. Harmful Algae 29 (2013) 31–39.

40. 油茶:Establish a cDNA-AFLP Technology System in Camellia oleifera. Molecular Plant Breeding, 2013, Vol.11, No.5, 611-616.

41. 亚洲百合:Transcriptomic analysis of Asiatic lily in the process of vernalization via RNA-seq. Mol Biol Rep. DOI 10.1007/s11033-014-3250-2.

42. 毛泡桐:Dynamic expression of novel and conserved microRNAs and their targets in diploid and tetraploid of Paulownia tomentosa. Biochimie xxx (2014) 1e10.

43. 人参:Cloning and Sequence Analysis Squalene Epoxidase Gene in Panax gin-seng. Journal of Jilin Agricultural University 2014, 36(2): 149-152,17

44. 雪莲:Cloning and Sequence Analysis of rbcs Gene from Sasussured involucrdta Kar. et Kir. Chinese Agricultural Science Bulletin 2014, 30(15): 261-267

45. 柑橘3Secreted Expression of Cecropin B Gene Enhances Resistance to Xanthomonas axonopodis pv. citri in Transgenic Citrus sinensisTarocco Acta Horticulturae Sinica 2014, 41(3): 417428 http: // www. ahs. ac. cn

46. 菊花:Stem apex detoxification culture markedly improved severalphysiological characters of chrysanthemum ‘YUTAI’. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0541-1

47. 荞麦和拟南芥:Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant. Gene 2014, 550(2): 200–206

48. 油松:Differential expression of SLOW WALKER2 homologue in ovules of female sterile mutant and fertile clone of Pinus tabulaeformis. Russian Journal of Developmental Biology 2014, 45(2): 78-84

49. 玫瑰花:Precise spatio-temporal modulation of ACC synthase by MPK6 cascade mediates the response of rose flowers to rehydration. The Plant Journal 2014, 79(6): 941–950

50. 棉花和拟南芥:Functional characterization of GhAKT1, a novel Shaker-like K+ channel gene involved in K+ uptake from cotton (Gossypium hirsutum). Gene 2014, 545(1): 61–71

51. 棉花和拟南芥1Upland Cotton Gene GhFPF1 Confers Promotion of Flowering Time and Shade-Avoidance Responses in Arabidopsis thaliana. PLoS ONE 2014, 9(3): e91869. doi:10.1371/journal.pone.0091869

52. 白杨:Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0536-y

53. 毛果杨:Molecular characterization of the SPL gene family in Populus trichocarpa. BMC Plant Biology 2014, 14: 131

54. 葛根:Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata. Plant Cell Reports 2014, 33(7), 1173–1185

55. 百合:Cloning and Expression Analysis of Actin GenelilyActinfrom Lily. Acta Horticulturae Sinica 2013, 40(7): 13181326

56. 百合1 Vernalization of Oriental hybrid lily ‘Sorbonne’: changes in physiology metabolic activity and molecular mechanism.Molecular Biology Reports 2014, DOI 10.1007/s11033-014-3545-3

57. 黄鹌菜:Transcriptome Sequencing and De Novo Analysis of Youngia japonica Using the Illumina Platform. PLoS ONE 2014, 9(3): e90636. doi:10.1371/journal.pone.0090636

58. 棉花1Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers. PLoS ONE 2014, 9(5): e96537. doi:10.1371/journal.pone.0096537

59. 苹果:Low Medium pH Value Enhances Anthocyanin Accumulation in Malus Crabapple Leaves. PLoS ONE 2014, 9(6): e97904. doi:10.1371/journal.pone.0097904

 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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