双荧光素酶报告基因检测试剂盒上海新睿生物科技有限公司

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产品名称: 双荧光素酶报告基因检测试剂盒
英文名称: SuperLight™ Dual Luciferase Reporter Gene Assay Kit
产品货号: SLDL-100
产品规格: 100次检测
产品产地: 美国
产品商标: BioAssay Systems
价    格: 2146元
保存与运输: -20℃
产品资料: 资料下载
更新时间: 2018/1/22 10:09:00
浏览人数:15
诚信指数:65点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

上海新睿生物科技有限公司
地址:
上海市徐汇区蒲汇塘路11号博大商务楼1307室
邮编:
200030
电话:
021-64288052 13818234547
传真:
联系人:
刘君
所在区域:
上海·上海
邮件:
公司展台:
在线QQ:
我是20434417,请问我有什么可以帮到您呢?

产品详细描述

 Application
• Bioluminescent reagent system for rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells.

Key Features
• • High sensitivity and wide detection range: detection of as little of 2 fg luciferase. 

• Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, and measured with any luminometer. Can be readily automated on HTS liquid handling systems.

• Fast and convenient: three step assay allows detection of dual luciferase levels within 20 minutes. 

Method
• Luminescence

Samples
• Cells etc

Species
• All

Procedure
• 20 min

Size
• 100 tests

Detection Limit
• 2 fg luciferase

Shelf Life
• 12 months

More Details
• The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The SuperLight™ Dual-Luciferase Reporter Gene Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates.

订货信息

SLDL-100       双荧光素酶报告基因检测试剂盒           100次检测          ¥2146

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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