Human TrkB PicoKine™ ELISA Kit上海新睿生物科技有限公司

性能参数

产品名称: Human TrkB PicoKine™ ELISA Kit
英文名称: Human TrkB PicoKine™ ELISA Kit
产品货号: EK0848
产品规格: 96T
试剂级别: 分析纯
产品产地: 武汉博士德
产品商标: Boster
价    格: 2000元
保存与运输: 2-8度
产品资料: 实验方法技术资料
更新时间: 2019/6/3 12:59:00
浏览人数:45
诚信指数:66点
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使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

上海新睿生物科技有限公司
地址:
上海市徐汇区蒲汇塘路11号博大商务楼1307室
邮编:
200030
电话:
021-64288052 13818234547
传真:
联系人:
刘君
所在区域:
上海·上海
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产品详细描述

 

  • 产品概况

    产品名称 Human TrkB ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TrkB
    引用此产品 Human TrkB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK0848)
    验证种属 Human
    免疫原 标准品表达系统:NSO,C32-H430
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml 
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 156pg/ml-10000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates and tissue homogenates. *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    一般表达浓度 以下文章提供了NTRK2的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    - 96-well plate precoated with anti- human TrkB antibody 1
    ST0000-10 Lyophilized recombinant human TrkB standard 10ng/tube??2
    AR1107 Biotinylated anti- human TrkB antibody 130??l(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130??l(dilution 1:100)
    AR1106-1 Sample diluent buffer 30 ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    - Adhesive cover 4
     
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 NTRK2
    蛋白名 BDNF/NT-3 growth factors receptor
    基因全名 BDNF/NT-3 growth factors receptor
    蛋白功能 Receptor tyrosine kinase involved in the development andthe maturation of the central and the peripheral nervous systemsthrough regulation of neuron survival, proliferation, migration,differentiation, and synapse formation and plasticity. Receptorfor BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is lessefficient in activating the receptor but regulates neuron survivalthrough NTRK2. Upon ligand-binding, undergoes homodimerization,autophosphorylation and activation. Recruits, phosphorylatesand/or activates several downstream effectors including SHC1,FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlappingsignaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates theGRB2-Ras-MAPK cascade that regulates for instance neuronaldifferentiation including neurite outgrowth. Through the sameeffectors controls the Ras-PI3 kinase-AKT1 signaling cascade thatmainly regulates growth and survival. Through PLCG1 and thedownstream protein kinase C-regulated pathways controls synapticplasticity. Thereby, plays a role in learning and memory byregulating both short term synaptic function and long-termpotentiation. PLCG1 also leads to NF-Kappa-B activation and thetranscription of genes involved in cell survival. Hence, it isable to suppress anoikis, the apoptosis resulting from loss ofcell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediatecommunication between neurons and glia..
    Tissue Specificity Isoform TrkB is expressed in the central andperipheral nervous system. In the central nervous system (CNS),expression is observed in the cerebral cortex, hippocampus,thalamus, choroid plexus, granular layer of the cerebellum, brainstem, and spinal cord. In the peripheral nervous system, it isexpressed in many cranial ganglia, the ophthalmic nerve, thevestibular system, multiple facial structures, the submaxillaryglands, and dorsal root ganglia. Isoform TrkB-T1 is mainlyexpressed in the brain but also detected in other tissuesincluding pancreas, kidney and heart. Isoform TrkB-T-Shc ispredominantly expressed in the brain..
    Subcellular Localization Cell membrane; Internalized to endosomesupon ligand-binding..
    Uniprot ID NTRK2: Q16620
    指标别名 BDNF/NT-3 growth factors receptor; 2.7.10.1; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; NTRK2; TRKB;

    BDNF/NT-3 growth factors receptor指标简介

    TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "e;neurotrophins"e;, which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "e;tyrosine kinase"e; is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "e;substrates"e;.Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3.

    批内差/批间差

      Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 202 1009 4057 195 887 3332
    Standard deviation 12.72 54.48 198.73 14.62 54.10 193.25
    CV(%) 6.3 5.4 4.9 7.5 6.1 5.8

    Human TrkB ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30min)
    Concentration(pg/ml) 0 156 312 625 1250 2500 5000 10000
    O.D. 0.009 0.084 0.149 0.269 0.459 0.818 1.530 2.212
    *典型数据仅供参考。用户需要自己建立标准曲线。

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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