CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem™ hPSC Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem™ hPSC Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem™ hPSC Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.
CryoStem™ hPSC Freezing Medium contains methylcellulose and DMSO in place of serum. The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.
CryoStem™ hPSC Freezing Medium Features：
Chemically defined, Animal component-free (ACF), Protein-free
Works with various media
Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
High recovery efficiency: maintains excellent attachment ability as well as growth performance
Maintains hESC and hiPSC pluripotency
Complete formulation; Ready-to-use at 2-8°C
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