Potassium Chloride 0.075 M is a hypotonic solution, utilized for the preparation of blood lymphocyte chromosomes. Hypotonic treatment with
KCl, often with the addition of Sodium Citrate is used most often to enhance membrane permeability and induce hypotonic cell swelling at
either room temperature (15-30°C) or 37°C. Usually, the higher temperature is used to increase metaphase spreading. There are several factors that influence the extent of the hypotonic treatment utilizing KCl and/or Sodium Citrate:
¨ Cell Density
¨ Specimen Type(e.g. slides or cell pellet)
It is important to note that the type, extent and application of hypotonic treatment is determined for the most part, empirically, in the laboratory
by trial and error and therefore may need to be modified from time to time to meet particular requirements and/or applications.
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