LightCycler® Fluorescein CPG   

LightCycler ® 荧光素 CPG

3 Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments.

在所有 LightCycler ®  480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3 染料。

For life science research only. Not for use in diagnostic procedures.

仅用于生命科学研究。不用于诊断过程。

Product No：03138178001   产品编号：03138178001  

Pack Size：1g   包装尺寸：1克

Keywords: Other qPCR Consumables  关键词：其他 qPCR 耗材

Overview概述：

Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3 terminus of oligonucleotides.

用于标记寡核苷酸 3 末端的荧光素长链烷基氨基控制孔玻璃（lcaa CPG，500 Å）。

Contents 内容

white solid

白色固体

Application应用：

LightCycler® Fluorescein CPG labels the 3 end of an oligonucleotide that is combined with a 5 LightCycler® Red-laeled oligonucleotide when using HybProbe probes for detection. This support can be used in the same manner as standard nucleoside supports since it contains a dimethobxytrityl group.

当使用 HybProbe 探针进行检测时，LightCycler ® 荧光素 CPG 标记寡核苷酸的 3 末端，该寡核苷酸与 5 LightCycler ®  Red-laeled 寡核苷酸结合。该支持物可以以与标准核苷支持物相同的方式使用，因为它含有二甲氧基三苯甲基。

Detail 细节

The LightCycler® Fluorescein CPG is derived from a pure dye isomer, and is attached via a linkage that is stable during oligonucleotide synthesis. Therefore, HPLC purification is simple and results in a well-defined product. HybProbe probes synthesized using the LightCycler® Fluorescein CPG are blocked at the 3 terminus, which prevents them from Taq DNA polymerase extension and exonuclease digestion. Thus, the resulting oligonucleotide is perfectly suited for mono- and dual-color experiments using the LightCycler® Carousel-Based System Instruments.

LightCycler ® 荧光素 CPG 源自纯染料异构体，并通过在寡核苷酸合成过程中稳定的连接键连接。因此，HPLC 纯化很简单，可以得到明确定义的产品。使用 LightCycler ® 荧光素 CPG合成的 HybProbe 探针在 3 末端被封闭，从而阻止它们进行 Taq DNA 聚合酶延伸和核酸外切酶消化。因此，所得寡核苷酸非常适合使用 LightCycler ® 基于旋转木马的系统仪器进行单色和双色实验。

Spectral characteristics for an oligonucleotide labeled with LightCycler® Fluorescein:

Excitation maximum: 494 nm

Emission maximum: 519 nm (both measured in 2 mM Tris buffer, pH 8.3).

用 LightCycler ® 荧光素标记的寡核苷酸的光谱特征：

最大激发波长：494 nm

发射最大值：519 nm（均在 2 mM Tris 缓冲液，pH 8.3 中测量）。