RN38-EASYspin Plus 植物RNA快速提取试剂盒-核酸纯化-试剂-生物在线
RN38-EASYspin Plus 植物RNA快速提取试剂盒

RN38-EASYspin Plus 植物RNA快速提取试剂盒

商家询价

产品名称: RN38-EASYspin Plus 植物RNA快速提取试剂盒

英文名称: EasySpin plus plant RNA rapid extraction kit

产品编号: RN3802

产品价格: 0

产品产地: 国产

品牌商标: 艾德莱

更新时间: null

使用范围: null

杭州昊鑫生物科技股份有限公司
  • 联系人 :
  • 地址 : 江干区天城路176号白云大厦2幢1003
  • 邮编 :
  • 所在区域 : 浙江
  • 电话 : 159****9305
  • 传真 : 0571-86955191
  • 邮箱 : 351778830@qq.com

产品介绍:

     本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶,植物RNA助提剂PLANTaid帮助结合多糖多酚并通过离心去除,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,基因组DNA清除柱子同时吸附清除残留的DNA, 然后RNA被选择性洗脱滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点:

1.完全不使用有毒的苯酚,氯仿等试剂,也不需要乙醇沉淀等步骤。
2.简捷,单个样品操作一般可在25分钟内完成,世界上最简单快速的试剂盒。
3.独有的植物RNA助提剂可以有效结合多糖多酚,提高清除效果。
4.独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。
5.适应性极其广泛,可以提取包括棉花、松针、冬青树叶、葡萄叶片、等100多种国内外试剂盒提取失败的样品。详细样品列表请参考公司主页产品介绍。
6.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。
使用EASYspin/EASYspin Plus试剂盒已经有大量文章发表,请联系我们索取发表的文章和包括北京林业大学,北京农学院,中国农业大学,农林科学院果树研究所,林业所,中国农科院等大学和研究院所大量使用案例。

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2.     樱桃花、叶、颚等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium    L.)    causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December    2012

3.     洋葱根、茎、蕾、叶、雌雄蕊等各部位Cloning and Expression Analysis of A Putative B Class MADS-box    Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769

4.     芜菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid       Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January    2012,    Pages 47–59

5.        1 EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H

6.     番茄叶:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science  Bulletin 2012,28(10):132-135

7.     栀子叶:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural    Science Bulletin.2012, 28(27):194-198

8.     油桐果实:Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong.     Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding GeneVfBCCP)  from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August

9.     油桐果实1Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time    PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084

10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra    haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 3512):1814-1821

11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709

12. 石斛1ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705

13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012

14. 大豆1Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012

15. 红花玉兰:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5

16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104

17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online15 March, 2013

18. :五倍子1Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9

19. 青杄 cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401

20. 青杄 1cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206

21. 青杄 2Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49No. 10, Oct. 2013.

22. 洋葱:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y

23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17.

24. 木瓜1Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C:

25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x

26. 柑橘1Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 18.

27. 柑橘2 Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.

28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.

29. 栀子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198

30. 丹参:Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.

31. 牡丹:Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996

32. 东南景天:Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165.

33. 山苍子:Identification of appropriate reference genes for normalizing transcript expression by quantitative realtime PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1

34. 木本植物:Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0

35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.