Stable cell line generation北京安必奇生物科技有限公司

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技术服务信息

服务名称:Stable cell line generation
服务类别:分子生物学服务 - 细菌菌株构建服务
价格:Inquiry元
允许参观:不允许参与实验
所在区域:北京.北京
更新时间:2018/5/8 18:50:00
浏览人数:2254
诚信指数:964点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

供应商联系卡

北京安必奇生物科技有限公司
地址:
北京市通州区经海五路1号院22号楼
邮编:
电话:
400-008-6094
传真:
联系人:
章文羿
所在区域:
北京·北京
邮件:
公司展台:
在线QQ:
我是1852676992,请问我有什么可以帮到您呢?
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技术服务详细描述

 Creative Biogene offers stable cell line development using an innovative and unique platform that allows one to go from gene to stable cell line. Based on many years' experience on cell line development, Creative Biogene's cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements.  Creative Biogene provides commercial and research cell line based on its proprietary expression vector, high throughput cell screening technologies and serum, animal components free process without any adaptation issue in a significantly reduced timeline.  Creative Biogene has developed innovative platforms ″Lenti-Stable″ (stable cell line using lentivector) and ″Select-Stable″ (stable cell line using selection system) to provide a rapid and cost-effective stable cell line service. Stable cell line is the best solution, not only to manufacture therapeutic and diagnostic protein, but also for application in drug screenings, as well as pharmacological and toxicological studies. Depending on the type of gene expression and the gene construct, there are many different approaches for establishing stable cell lines. Development of stable cell lines capable of producing recombinant proteins and monoclonal antibodies at high and reliable levels can be critical to a biopharmaceutical research project. The basis of manufacturing biologically active products relies on generating high quality cell lines which comply with current regulatory guidelines.  Creative Biogene platform provides a rapid and cost-effective solution for stable cell line generation, which can be used for cellular function assays, trafficking, protein production, target validation, and other research purposes.  Creative Biogene's expert staff and state-of-the-art facilities are fully equipped to execute complete studies on genetic and productive stability in the presence and absence of selective agents.  Creative Biogene "Select-Stable" platform uses Glutamine Synthetase (GS) or DihydrofolateReductase (DHFR) systems to construct the stable cell lines.

Creative Biogene  offers cell line development services to accommodate a variety of stages of the drug development process as follows: 
 • Cloning of the provided cDNA or your gene of interest into a suitable expression vector. Codon
    optimizations can be introduced for the high yield production.
 • Transfection of host cell line which is compliance with FDA guideline. 
 • Incubation and cell line recovery/growth.
 • Colony isolation, clone screening using ELISA, Western, or cell based assay of your interest, such as
   intracellular calcium, IP1, intracellular cAMP, thallium flux or membrane potential assay, et al.
 • Stable cell line expansion.
 • Cell line and supernatant harvest & processing.
 • Characterization and assay according to your requirements (optional).
 • Free cell line backup service for two years from delivery of cell clones.

Creative Biogene "Lenti-Stable" platform engineers in house lentiviral vector for high efficient gene integration into cell genome with extremely low false positive clones.  Creative Biogene offers cell line development services to accommodate a variety of stages of the drug development process as follows:  • Construct your gene into our lentiviral vector with desired selection marker.

 • Generate the lentivirus particles. 
 • Sequentially transduce the cell line of your interest.
 • Select the clones constantly expressing your gene at high level.
 • Validation the genomic integration of gene via genomic PCR.
 • Validation the high-expression clone by Western Blot (if applicable).
 • Deliverable: Two cryogenic preserved vials of stable cells and final report. 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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