Colcemid

MCE 国际站：Colcemid

CAS：477-30-5

品牌：MedChemExpress (MCE)

存储条件：Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.

生物活性：秋水仙碱 (Demecolcine) 是秋水仙碱的衍生物，是一种有效的有丝分裂抑制剂^[1][2]。秋水仙碱与蛋白质、微管蛋白结合并使细胞停滞在中期以进行核型分析。秋水仙碱可诱导细胞凋亡，可用于肿瘤研究^[2]。

体外：Colcemid (Demecolcine) (0.1-0.25 μg/ml, 1小时) 降低小鼠、大鼠和青蛙精母细胞的分裂 II 期补体的低倍性频率[3]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内：Colcemid (Demecolcine) (0.3 mg/kg，腹腔注射) 在经 Demecolcine 腹腔注射处理后的小鼠中提高了分裂 II 期低倍性的发生率[3]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Mice[3] Dosage: 0.3 mg/kg Administration: Intraperitoneal injection (i.p.) Result: Elevated 7.8-8-fold of the hypoploid and hyperploid frequencies in metaphase II cells relative to controls.

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研究领域：Cell Cycle/DNA Damage  |  Cytoskeleton  |  Apoptosis

作用靶点：Microtubule/Tubulin  |  Apoptosis

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参考文献：

[1]. T Tsuchida, et al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31(4):295-9.[2]. Ashley M Rozario, et al. Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy. Bioexiv.[3]. Nett RS, et al. Discovery and engineering of colchicine alkaloid biosynthesis. Nature. 2020 Aug;584(7819):148-153. doi: 10.1038/s41586-020-2546-8. Epub 2020 Jul 22. Erratum in: Nature. 2020 Jul 30.[4]. Muzaffar A, et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J Med Chem. 1990 Feb;33(2):567-71.[5]. Risley MS, et al. An improved method for cytogenetic analysis of meiotic aneuploidy in rodent and frog spermatocytes. Mutat Res. 1990 Dec;234(6):361-8.