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A protocol for a lung neovascularization model in rodents
Rosemary C Jones, Diane Capen, Bodil Petersen, Rakesh K Jain, Dan G Duda
SUMMARY: By providing insight into the cellular events of vascular injury and repair, experimental model systems seek to promote timely therapeutic strategies for human disease.
CONTEXT: ...12-548-B) Formvar film/copper grids 200 mesh (EMS, cat. no. FF200-CU) Dumont N-5 tweezers (EMS, cat. no. 72854-D) NUNC 245 mm square bioassay dish (Fisher Scientific, NNI no. 240835) Tissue-culture Petri dish, 150 mm 25 mm...
Nature Protocols 3, 378 - 387 (01 Mar 2008) Protocols
A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents
Rosemary C Jones, Diane E Capen, Kenneth S Cohen, Lance L Munn, Rakesh K Jain, Dan G Duda
SUMMARY: The goal of many current studies of neovascularization is to define the phenotype of vascular cell populations of different origins and to determine how
CONTEXT: ...200-square mesh, thin bar, high definition (EMS, cat. no. FF200-NI) Dumont N-5 tweezers (EMS, cat. no. 72854-D) Nunc 245 mm square bioassay dish (Fisher Scientific, cat. no. NNI 240835) Tissue-culture Petri dish, 150 mm 25 mm...
Nature Protocols 3, 388 - 397 (01 Mar 2008) Protocols
Mechanisms of acquired resistance to cetuximab: role of HER (ErbB) family members
D L Wheeler, S Huang, T J Kruser, M M Nechrebecki, E A Armstrong, S Benavente, V Gondi, K-T Hsu, P M Harari
SUMMARY: The epidermal growth factor receptor (EGFR) is a central regulator of proliferation and progression in human cancers. Five EGFR inhibitors, two monoclonal antibodies and three TKIs, have recentl
CONTEXT: ...staining of EGFR Cells (5000 cells per well) were seeded on a four-well glass chamber slide (Nalgene Nunc, Naperville, IL, USA). Following EGF stimulation, cells were washed with PBS and fixed with 4% paraformaldehyde for 10 min....
Oncogene (25 Feb 2008) Original Article
Large-scale chemical dissection of mitochondrial function
Bridget K Wagner, Toshimori Kitami, Tamara J Gilbert, David Peck, Arvind Ramanathan, Stuart L Schreiber, Todd R Golub, Vamsi K Mootha
SUMMARY: Mitochondrial oxidative phosphorylation (OXPHOS) is under the control of both mitochondrial (mtDNA) and nuclear genomes and is central to energy homeostasis. To investigate how
CONTEXT: ...all screening, 4,000 C2C12 myoblasts per well were seeded into either black or white 384-well optical-bottom plates (Nunc) at 50 l per well. On day 4 of differentiation, 100 nl of each compound was pin-transferred in duplicate into...
Nature Biotechnology (24 Feb 2008) Research
Efficient tuberculosis treatment in mice using chemotherapy and immunotherapy with the combined DNA vaccine encoding Ag85B, MPT-64 and MPT-83
D-H Yu, X-D Hu, H Cai
SUMMARY: Although most cases of tuberculosis (TB) can be cured with antibiotics, relapse is common if patients do not continue chemotherapy for at least 6 months. Thus, improved therapeutic strategies ar
CONTEXT: ...were adjusted to a concentration of 4 × 106 cells ml−1 before being cultured in round-bottomed microwell plates (Nunc, Roskilde, Denmark) in RPMI-1640 medium supplemented with L-glutamine, 2-mercaptoethanol (50 μM), penicillin (100 U...
Gene Therapy (21 Feb 2008) Original Article